Despite Shakhtar being without a number of key players for the game in Russia, Spalletti has told his players not to take any chances.
"They are without [Dmytro] Chygrynskiy, Fernandinho and Jadson but that does not weaken Shakhtar," Spalletti told the press.
"The problem Lucescu has is that he must leave some good players out. And those players who get a chance to start will be very motivated to prove their skill and class.
"I know Lucescu, he's an old football wolf, noble and wise, but look at our logo – there is a lion. Now we will see who is stronger: a lion or a wolf."
Zenit are second in the group on four points, one behind leaders APOEL Nicosia. Shakhtar are bottom with just two points from three games.
Kick-off in St Petersburg is at 18.00CET.
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